The Azurescens Foundation - Growing Psilocybe azurescensby
The Azurescens Foundation
polish transl. http://www.psilosophy.info/eneiegggatblarbuceadczbe
original source: http://www.magic-mushrooms.net/growing-psilocybe-azurescens.pdf
backup source: http://www.psilosophy.info/resources/growing-psilocybe-azurescens.pdf
Table of Contents:
Maltextract agar can easily be prepared in a common kitchen. The most expensive thing needed is a pressure cooking pot. The medium must be sterilised (autoclaved), or you will grow nothing but mold.
Fill the medium in suitable containers suck as jars of not more than 10cm height (the smaller the better to handle).
Pour about lcm of medium in the jars. The jars are covered with the caps, but do not screw them tight, or the jars will break during cooking!!! The caps can additionally be covered with tin foil to minimize the risk of airborne contamination.
The jars are placed in the pressure cooking pot filled with several cms of water.
This is hot enough, if maintained for 20 minutes, to kill any spores of mold and bacteria. After this time period let the pot cool to room temperature.
Short: Place some of the mushroom spores on sterile maltextract agar. Let the glass stand at room temperature (18 to 24°C/65 to 75°F). The spores will germ within a few days. Three weeks later the mycelium should be visible as cottony white hairs growing in all directions from where the spores had been placed.
How to transfer spores to maltextract agar in a sterile way:
Now it is time to take a little piece of white mycelium and put it in a fresh jar with agar medium.
Sometimes this isolation of pure medium has to be repeated several times until a really pure culture free of any contaminants is obtained A contamination is anything that grows on your medium that is not the mycelium.
From a pure culture the mycelium can be propagated further. A very good wood substrate are fresh hard wood chips presoaked in water.
Excellent for the expansion of Psilocybe azurescens:
Put them in as big glass jars as fit in your pressure cooking pot. The jars should only be filled to two third, this allows you to shake the wood chips in the jars.
The procedure for sterilization is exactly the same as for the preparation of malt extract agar, except for the cooking time which must be increased to a full hour or more at max. pressure, depending on the container size.
As soon as the jars have cooled clown to room temperature they can be inoculated with several pieces of agar culture or if available wood spawn.
A few days later growth of the mycelium should be visible.
The wood an a the mycelium form a compact mass of a white colour witch produces a characteristic mushroom odor when broken up. This mass of mycelium, suitable for the preparation of an outdoor bed, is called spawn.
Instead of jars, heat resistant polypropylene bags can be used to expand the mycelium to the wished volume, but the risk of contaminations is higher.
Do not use contaminated cultures to inoculate new Substrate!!!
An outdoor bed is nothing else than a 15 to 20cm deep bole m the soil filled with wood substrate.
The bed is watered with several liters depending on its size. The patch can additionally be covered with wet cardboard and perforated plastic or anything that keeps moisture high.
Do not give to much water! It is better to repeat watering a week later than to give too much first.
Make sure the bed is humid the first two weeks but not wet, then you will not have to give any more water until September. If you overwater during summer, the mycelium may grow aggressively, but you will probably see no mushrooms in late autumn.
From September on keep the patch wet by watering once or twice a week.
The cardboard, the bed is covered with, is itself used by the mushroom as medium. A new bed of wood chips could be inoculated just by placing the infected cardboard on a small layer of wood chips at the bottom of the new bed, and by covering the cardboard with wood chips.
When the temperatures drop below 0°C the mushrooms stop growing. A layer of new wood chips can be worked in the top of the patch; this is the nutrition for the next year.
Treated that way, a mushroom bed may last for decades and zillions of spores will be set free.
This species originates from the Columbia river basin near Astoria, Oregon and was first found in 1979. It grows in beds of hardwood or conifer chips in half shaded environments.
It belongs to the so called Psilocybe cyanescens complex. This means it is closely related to Psilocybe cyanescens and Psilocybe cyanofibrillosa.
Psilocybe azurescens is distinguished by its comparatively great size and some microscopic characteristics.
Typical analysis of dried Psilocybe azurescens (%) (Gartz l995):
These data make clear, that Psilocybe azurescens is able to accumulate as high levels of tryptamine alkaloids as 25 milligram, per gram of dried mushrooms.
With these values it is the mushroom with the highest alkaloid concentrations, of all psilocybin mushrooms known so far.
Latin description published by Stamets and Gartz in 1995:
Psilocybe azurescens Stamets & Gartz sp. nov.: Pileo ochreato-brunneo, hygrophano, viscido, pellicula separabili intructo, conico dein convexo, plano 30-100 mm lato, umbonato.